Factors relating to expression of <i>IGF2R</i>, <i>IFNΤ</i>, <i>AKR1B1</i>, and <i>PLAC8</i> at the blastocyst stage.

<p>Relationship between <i>IGF2R</i> and timing of the first cleavage (<b>A</b> and <b>B</b>), <i>IFNΤ</i> and timing of the first cleavage (<b>C</b> and <b>D</b>), <i>IFNΤ</i> and the number of blastomeres at the end of the first cleavage (<b>E</b>), <i>AKR1B1</i> and the presence or absence of multiple fragments at the end of the first cleavage (<b>F</b>), and <i>PLAC8</i> and oxygen consumption at the blastocyst stage (<b>G</b> and <b>H</b>) are shown. Relative expression is shown as the fold change from the mean value of <i>in vivo</i>–derived blastocysts, which was set at 1. **Coefficient of determination, <i>r</i>, was statistically significant based on simple regression analysis (<i>P</i><0.01) (<b>A</b>, <b>C</b>, and <b>G</b>). Different letters indicate significant differences between groups based on one-way ANOVA followed by Fisher's PLSD (^A,B<i>P</i><0.01) (<b>B</b>, <b>D</b>, <b>F</b>, and <b>H</b>). Gene expression in blastocysts classified based on timing, blastomere number, the presence or absence of multiple fragments at the end of the first cleavage, and oxygen consumption of blastocysts at 168 hpi (<b>I</b>). Embryos displaying similar patterns of expression for these five genes were grouped together on closely connected branches of the dendrogram with the same colors. The color map indicates normalized expression values that were based on <i>in vivo</i>–derived blastocysts for each gene examined. Red represents high expression; black, approximately equal expression; and green, low expression as compared with <i>in vivo</i>–derived blastocysts.