<i>In vitro</i> characterization of BAC cells transduced simultaneously with p53^DD and Ras^G12V.

<p>A, summary of the experimental design for the generation of stably infected cell populations. B, detection of Ras^G12V and p53^DD by RT-PCR in adrenocortical PR, P, R and pL cells. Total RP-L27 acts as loading control. C, Confirmation of protein expression by immunoblotting of PR, R, P and pL cells. Actin served as a loading control. D, growth curves of PR, R, P and pL cells. The <i>in vitro</i> cell proliferation rates were obtained by counting cells from triplicate cell culture dishes every day. <i>Points</i>, mean; <i>bars</i>, SD. E, asynchronous populations of BAC cells were cultured in normal culture medium or in defined medium containing 0.1% FCS. The percentage of cells engaged into the cell cycle was determined by measuring the Ki-67 labeling index. <i>Bars</i>, SD.