Mutations in the JAMM motif of CSN-5 partially restore SCF-mediated FRQ degradation in the <i>csn-5^KO</i> strain.

<p>(A) Western blot analyses showing degradation of FRQ protein in <i>csn-5</i> mutant and the different CSN-5 complementation strains after addition of cycloheximide (10 mg/mL). Asterisks indicate nonspecific bands detected by FRQ antibody. (B) Densitometric analyses from four independent experiments showing the degradation of FRQ in different strains.