ELK1 controls a network of actin/migration-related genes.

<p>(A) Network formed by proteins encoded by actin cytoskeleton/migration-related genes associated with “unique” ELK1-bound regions (each protein denoted by a circle). Asterisks mark genes tested in (B) (<i>PFN1</i> is associated with a redundant region). Grey circles indicate that gene expression is changed upon ELK1 depletion. See <a href="http://www.plosgenetics.org/article/info:doi/10.1371/journal.pgen.1002694#pgen.1002694.s011" target="_blank">Figure S11</a> for details of gene names. (B) mRNA levels of nine actin cytoskeleton- and migration-associated genes were measured by RT-qPCR in serum starved MCF10A cells transfected with siELK1 and normalised to an siGAPDH-transfected control; bars show average values from three biological repeats with standard deviations. Levels of <i>ELK1</i> mRNA indicate the efficiency of depletion; <i>RBL2</i>, <i>EFR3A</i> are negative controls which do not associate with ELK1. * P<0.05, ** P<0.01 (Student's paired t-test).