The Pab 1801 yields a cytoplasmic punctate pattern.

<p>U2OS, WI38, SK-N-SH, and HCT116 cells were fixed in PFA, permeabilized in Triton 0.1% and immunostained with the indicated antibodies against p53 as indicated in Materials and Methods. A, Under resting conditions, the Pab 1801 showed a cytoplasmic granular pattern, whereas the FL 393, the Pab 240, the Pab 421 and the Pab DO1 antibodies yielded a faint nuclear signal. B, WI38 cells were treated with daunorubicin or actinomycin D, and immunostained with the indicated antibodies. A strong p53 nuclear signal was observed in all cases. The Pab 1801 punctate signal in the cytoplasm remained unaltered. Bar, 10 µm.