Regulation of JA and SA associated maize marker genes in response to <i>U. maydis</i> wild type versus SG200Δpep1 infections.

<p>Expression levels of SA/JA marker genes were determined by quantitative real-time PCR. The expression values represent three biological replicates and are shown relative to <i>GAPDH</i> expression in each sample. Leaf samples of mock, SG200 or SG200Δpep1 infected plants were taken after 2 dpi. Expression levels in mock infected plants were set to 1 and relative expression of marker genes was calculated for SG200 (light grey bars) and SG200Δpep1 (dark grey bars) samples. (<b>A</b>) Expression of SA marker genes <i>atfp4</i>, <i>pox12</i> and <i>pr1</i> 24 hours after infection with strain SG200Δpep1 or SG200, respectively. (<b>B</b>) Expression of JA marker genes <i>cc9</i> and <i>bbi</i> after infection with strain SG200Δpep1 or SG200, respectively. Data represent three biological replicates. P values have been calculated by an unpaired <i>t</i> test. Error bars show SEM. * P≤0.05.