Characterization of BRITER cell line.

<p>(<b>A</b>) RT-PCR analysis of BRITER cell line for osteblast specific marker genes: Osterix (lane 1), ColIAI (lane 2), BSP (lane 3), Runx2 (lane 4), Osteocalcin (lane 5), Alkaline Phosphatase (lane 6), Large T antigen (lane 7) and GAPDH (lane 8), respectively. (<b>B</b>) Quantification of ALP activity of BRITER cell extracts cultured under indicated conditions. (<b>C</b>) Alkaline Phosphatase staining (<b>a–d</b>). Alizarin red staining (<b>e–h</b>). von Kossa staining (<b>i–l</b>) of BRITER cell line cultured under following conditions “−BMP−4 OHT” (<b>a, e, i</b>), “−BMP+4-OHT” (<b>b, f, j</b>), “+BMP+4 OHT” (<b>c, g, k</b>) and “+BMP−4-OHT” (<b>d, h, l</b>). Scale bar 10 mm. Data shown is means ± SEM of experiments carried out in triplicates.