Transgenic FGF1 expression and release in FGF1/Tek mice.

<p>A. FGF1 is expressed in kidney EC. Immunoperoxidase staining was used to detect transgenic FGF1 (anti-HA antibodies) and EC (anti-PECAM antibodies) in the paraffin sections of kidneys obtained from FGF1/Tek mice. Preparations were counterstained with hematoxylin. Bar –30 µ. B. Lysates of kidney tissue obtained from FGF1/Tek and control FVB mice were resolved by SDS-PAGE and immunoblotted using rabbit anti-FGF1 antibodies and mouse monoclonal anti-ß-actin antibodies (loading control). C. Transgenically expressed FGF1 is released into the vasculature of FGF1/Tek mice. Seven male FGF1/Tek mice and 7 control WT FVB males were sacrificed; their vasculatures were perfused with cold heparinized PBS and the content of FGF1 (ng/ml blood) was determined using an FGF1 ELISA kit from R&D.