Characterization of the decoy Wnt receptor sLRP6E1E2.

<p>(a) Schematic representation of the genomic structure of Ad vectors used. (b) Endogenous Wnt3a (left panel) and LRP6 (right panel) expression in several human lung cancer cell lines. (c) Secretion and expression of sLRP6E1E2. Cell culture supernatants were assessed with FLAG specific Ab (Upper panel). Ponceau staining is shown as loading control (Bottom panel). (d) H322 and H460 cells were transduced with dE1-k35/LacZ or dE1-k35/sLRP6E1E2 (50 MOI) for 48 hr. Cell lysates were immunoprecipitated with antisera against Wnt3a (IP: Wnt3a) or LRP6 (IP: LRP6) followed by western blot analysis with the same antibodies.