Decoy Wnt receptor sLRP6E1E2 induces apoptosis in human lung cancer cells.

<p>(a) Cells were transduced with dE1-k35/LacZ or dE1-k35/sLRP6E1E2 at (20 MOI), and photographs were taken at 72 hr later. Original magnification, ×200. (b) Detection of sLRP6E1E2-induced apoptosis by TUNEL staining. Original magnification, ×400. (c) Total number of TUNEL-positive cells per fields (mean ± SEM). *<i>P</i><0.05 versus PBS or dE1-k35/LacZ treated with Wnt3a; **<i>P</i><0.001 versus PBS-treated or dE1-k35/LacZ-transduced controls. n.s. = not significant. (d) Western analysis of sLRP6E1E2-mediated apoptosis. H460 cells were transduced with dE1-k35/LacZ or dE1-k35/sLRP6E1E2 (20 MOI). The western blot using specific antibodies against uncleaved PARP, cleaved PARP, pro-caspase-3, cleaved caspase-3, and cytochrome <i>c</i>. (e) H460 cells were treated as indicated above (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0036520#pone-0036520-g004" target="_blank">Fig. 4d</a>). Subcellular localization of cytochrome <i>c</i> was determined by western blot analysis of cytosolic and microsomal fractions.