Wnt3a treatment results in the disruption of cell-cell junctions and epithelial-to-mesenchymal transition in tumor cells.

<p>(a) H322 cells were treated with Wnt3a (100 ng/ml) for the indicated times, and morphology changes were observed by light microscopy. Original magnification, ×200. (b) E-cadherin, Vimentin, and β-catenin mRNA levels in H322 cells after Wnt3a treatment. (c) H322 cells were stained with DAPI (blue), TRITC-labeled actin (red), or anti E-cadherin (green) after 24 incubation with or without Wnt3a (100 ng/ml). Original magnification, ×630.