Analysis of NRG-induced autophagy compared to Rapamycin-mediated autophagy.

<p>(A) LNCaP cells were treated with either 100 ng/ml NRG or 50 nM rapamycin (Rapa) for 24 h, in the presence or in the absence of 10 mM NAC. Whole cell lysates were prepared and subjected to an immunoblot analysis with anti-LC3, anti-phospho-S6K and anti-S6K antibodies. (B) Densitometric analysis of the results described in A is represented as fold induction of the control untreated cells (n = 3, means ± S.D). (C) Representative images of cell morphology following treatments with NRG and rapamycin are shown (Olympus, 20×magnification). (D) LNCaP cells were treated with either 100 ng/ml NRG, 50 nM rapamycin or both for 24 h. Whole cell lysates were prepared and subjected to an immunoblot analysis with anti-LC3 antibodies. This experiment was repeated three times with similar results.