Creation of BRITER Cell Line.

<p>(<b>A</b>) Schematic showing steps involved in the creation of BRITER Cell Line. (<b>B</b>) Schematic showing critical genetic components of BRITER Cell Line. (<b>C</b>) BMP2 dependent FFLuc activity of BRITER Cell Line under four different conditions, namely “−BMP. −4-OHT”, “−BMP, +4-OHT”, “+BMP, +4-OHT” and “+BMP, −4-OHT”. (<b>D</b>) Anti-PSMAD 1/5/8 immunofluorescence in BRITER cell line under four different conditions: (<b>a</b>) −BMP−4-OHT, (<b>b</b>) −BMP+4-OHT, (<b>c</b>) +BMP+4-OHT and (<b>d</b>) +BMP−4-OHT. Scale bar 100 µm. (<b>E</b>) Quantification of Anti-PSMAD 1/5/8 immunofluorescence by Image J. (<b>F</b>) Western blot analysis of BRITER cell extracts cultured under indicated conditions with PSMAD 1/5/8 antibody. β-tubulin antibody has been used as loading control. Data shown are means ± SEM of three independent experiments carried out in triplicates.