PITX2 elevates the expressions of <i>Cyclin-D1</i> and <i>C-myc</i> in ovarian cancer cells.

<p>(<b>A</b>) Q-PCR analysis revealed that the levels of <i>Cyclin-D1</i> and <i>C-myc</i> were elevated in PITX2A stably expressing clones (C4, C5 of SKOV-3 and C33, C34 of OVCA 433). (<b>B</b>)Western blot analysis showed that the protein levels of Cyclin-D1 and C-myc were increased by PITX2 in PITX2A stably expressing clones (C4, C5 of SKOV-3 and C33, C34 of OVCA 433). (<b>C</b>) Knockdown of PITX2 significantly reduced the levels of Cyclin-D1 and C-myc in PITX2 knockdown stable clones (C6, C8 of OVCA 433 and C1, C4 of OV2008). The levels of HA-tagged PITX2A were detected using anti-HA antibody, while endogenous PITX2 expression was examined by anti-PITX2 antibody, β-actin was used as loading control. The numerical value under each panel represents the relative expression of Cyclin-D1 and C-myc to their vector controls.