PITX2 enhances anchorage-independent growth ability of ovarian cancer cells.

<p>(<b>A</b>)Enforced expression of PITX2A enhanced anchorage independent growth ability of ovarian cancer cells. The bar chart shows that PITX2A stably expressing clones (C33 and C34) of OVCA 433 (**<i>P</i> = 0.05) and (C4 and C5) of SKOV-3 (*<i>P</i> = 0.01) had increased number of colonies in soft agar compared with their vector controls. Representative pictures show larger colony size in PITX2A stable expressing clones under microscopy. (<b>B</b>)Depletion of PITX2 by shRNA inhibited anchorage independent growth ability of ovarian cancer cells. The number of colonies in PITX2stable knockdown clones (K1 and K2) of OVCA 433 and OV2008 showed significantly reduction as compared with the scrambled controls (* <i>P</i><0.01). Representative pictures shows that the reduced colony size of PITX2stableknockdown clones and their scrambled controls. The above experiment was repeated at least three times independently and the data was calculated with mean ± SD.