Effects of PKC inhibition on HG-induced IL-1β upregulation in BREC.

<p>(<b><i>A</i></b>) Effect of PKC activation on IL-1β expression. Confluent cultures of BREC (NG medium) were stimulated with PMA (10 and 30 nM) for 4 hours. Total RNA was extracted and IL-1β mRNA levels were assayed by quantitative RealTime RT-PCR. Bars represent mean ± SD of the results obtained in three different isolates tested in independent experiments. n = 9/group (<b><i>B</i></b>) Confluent cultures of BREC were exposed to NG, HG, or HG in the presence of the non-selective PKC inhibitors calphostin C (CalC, 50 mM) for 4 days. Total RNA was extracted and IL-1β mRNA levels were assayed by quantitative RealTime RT-PCR. Bars represent mean ± SD of the results obtained in three different isolates tested in independent experiments. n = 6/group.