Identification of major candidate mechanosensitive genes.

<p>Gene expression levels of compressed samples were compared to uncompressed controls. (A) DNA microarray analysis was performed on four independent pairs of uncompressed/compressed experiments. Expression level differences were sorted to identify highly responsive genes (fold change >2), resulting in a list of 20 transcripts. Bars represent the fold change in gene expression upon compression, i.e. up-regulation (red) or down-regulation (green) (p<0.01). Exact modulation factors and associated p-values are detailed in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0036964#pone-0036964-t001" target="_blank">Table 1</a>. (B) Real-time PCR analysis on three independent experiments confirmed DNA microarray results for eight selected genes. Bars represent the compression-induced gene expression modulation (mean +/− SD), either up-regulation (red) or down-regulation (green) (* p<0.05, ** p<0.01).