HC Analysis of the cytoplasmic area of U-2 OS cells treated with antimitotic compounds.

<p><b>A</b>) Representative segmentation images of control U-2 OS cells and epothilone B- or pretubulysin-treated cells that were acquired with 200-fold magnification on a BD Pathway 855 automated microscope and subsequently processed and analyzed in AttoVision v1.6.2. <b>B</b>) Representative results of HC analyses of the average cytoplasmic area of U-2 OS osteosarcoma cells treated for 24 h with antimitotic drugs at varying concentrations. Bars represent the mean ± SEM of all cellular segments within a well.