Analysis of U<sub>15</sub> binding activity of SpLsm2/3, SpLsm3, SpLsm5/6/7 and SpLsm4N.
- Donghui Wu (163745)
- Shimin Jiang (163751)
- Matthew W. Bowler (163758)
- Haiwei Song (163763)
Publication date
May 2012
DOIAbstract
<p>(A) Sensorgrams of surface plasmon resonance analysis using 5′-end biotin-labeled U15. Fluorescence anisotropy analysis using 5′-end FAM-labeled U15 showed that the fitted K<sub>d</sub> value of SpLsm2/3 is 4.0 ± 0.5 µM (B) and the fitted K<sub>d</sub> value of SpLsm5/6/7 is 52.5 ± 10.0 µM while no K<sub>d</sub> values could be determined for SpLsm3 and SpLsm4N (C).</p
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