Inhibition of phosphorylation of E3 ligases reduces KSHV entry and intracellular trafficking in endothelial cells.

<p>(A) Treatment with PP1 analog inhibits KSHV entry and intracellular trafficking in a dose-dependent manner. HUVEC pretreated with PP1 analog for 1 hr, infected with KSHV for 4 hr, and stained for KSHV particles (red) and nuclei (blue). (B) Quantification of the total number of Orf65+ particles docked at each nucleus following treatment with PP1 analog. (C) Treatment with PP1 analog prevents phosphorylation of c-Cbl during KSHV infection. HUVEC pretreated with Src kinase inhibitor PP1 analog for 1 hr were infected with KSHV for 30 min. E3 ligase c-Cbl, phospho-Y700 c-Cbl, and phospho-Y774 c-Cbl were detected using β-actin as a loading control. EGF was used as a positive control for c-Cbl activation.