DLF extracts causes cell growth inhibition and apoptosis.

<p>(A) The growth inhibition rates of Huh7, SMMC-7721, PLC/PFR/5 and L-02 cells resulting from treatment with ICD for 48 h. (B) Following the treatment of Huh7 cells with 0, 100, 200 or 300 µg/ml ICD for 48 h, apoptotic cells were detected by Annexin V and 7-AAD double staining. (C) Western blot analysis of cleaved PARP in Huh7, SMMC-7721 and PLC/PRF/5 cells following ICD treatment at their respective IC₅₀ values (250 µg/ml, 200 µg/ml, 250 µg/ml). (D) An amount of 1×10⁶ Huh7 or SMMC-7721 cells/mouse was subcutaneously injected into nude mice. After 2 weeks, 0.4 mg/ml of ICD or PBS was administered 5 times per week for 4 weeks. The resulting tumors were excised from the animals after treatment. (E) The tumor weights for the four groups of animals were compared, and statistical significance was determined using the Student’s <i>t</i>-test. Each point represents the mean ± SD.