Regulation of the endogenous RIP140 gene.

<p>(<b>A</b>) The levels of E2F1, cyclin D1 and RIP140 mRNAs were measured in MCF-7 cells 2 days after transient transfection with plasmids allowing the overexpression of the E2F1/DP1 heterodimer. Statistical analysis was performed using the Student t test (***p<0.0001). (<b>B</b>) HeLa cells were synchronized by 2 mM hydroxyurea (HU) and, after block release the distribution of cells in G₁, S and G₂/M was analyzed by flow cytometry (upper panel). Cyclin B1 and E, and RIP140 mRNA levels were quantified by real-time quantitative RT-PCR as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0035839#s2" target="_blank"><i>Materials and Methods</i></a> (lower panel). The results are expressed in arbitrary units after normalization by RS9 mRNA levels. Values are the means ± S.D. of three independent experiments.