Confined FGF2 expression eliminates high-level FGF2 associated anemia and ameliorates extramedullary hematopoiesis.

<p>(a) FGF2 negatively affects body weight. Mice were weighed at 8 weeks after transplantation. GFP control vs. β-globin-FGF2, not significant; GFP control vs. low SFFV-FGF2, <i>P</i><0.05; GFP control vs. medium SFFV-FGF2, <i>P</i><0.001; GFP control vs. high SFFV-FGF2, <i>P</i><0.001. (b) FGF2 negatively affects red blood cell count. Red blood cells in peripheral blood were counted at 8 weeks after transplantation. GFP control vs. β-globin-FGF2, <i>P</i><0.05; GFP control vs. low SFFV-FGF2, <i>P</i><0.05; GFP control vs. medium SFFV-FGF2, <i>P</i><0.01; GFP control vs. high SFFV-FGF2, <i>P</i><0.001. (c) FGF2 induces extramedullary hematopoiesis in a concentration-dependant manner. Shown are representative spleens and the spleen weight in different groups. GFP control vs. β-globin-FGF2, <i>P</i><0.05; GFP control vs. low SFFV-FGF2, <i>P</i><0.05; GFP control vs. medium SFFV-FGF2, <i>P</i><0.01; GFP control vs. high SFFV-FGF2, <i>P</i><0.001. (d) Flow cytometry analysis of CD45R⁺ B cells, CD3⁺ T cells, Gr-1⁺ and CD11b⁺ myeloid cells, and Ter119⁺ erythroid cells in spleens from the GFP control mice, the β-globin-FGF2 mice and the high SFFV-FGF2 mice at 8 weeks after transplantation. (e) Relative mRNA expression of <i>Spp1</i>, and <i>Cxcl12</i> in femurs from the GFP control mice, the β-globin-FGF2 mice and the high SFFV-FGF2 mice. For <i>Spp1</i>, GFP control vs. all the other groups, <i>P</i><0.001. For <i>Cxcl12</i>, GFP control vs. high SFFV-FGF2, <i>P</i><0.001; others, GFP control vs. other groups, not significant. Data shown are presented as mean ± SEM (n = 10 mice/group).