Effects of Dp-3-g on human platelet activation.

<p>Human PRP or gel-filtered platelets were incubated with control buffer, 0.5 µM Dp-3-g, 5 µM Dp-3-g or 50 µM Dp-3-g for 40 min at 37°C. Platelet activation markers were analyzed via flow cytometry after stimulation by ADP, collagen, TRAP or thrombin. A) P-selectin expression on human PRP. B) P-selectin expression on human gel-filtered platelets. C) CD63 expression on human gel-filtered platelets. D) CD40L expression on human gel-filtered platelets. Values are mean ± SEM, n = 3 per group. * <i>P</i><0.05, ** <i>P</i><0.01 and *** <i>P</i><0.001, as compared to control buffer.