Gastric colonization following oral infection with different <i>B. pseudomallei</i> isolates.

<p>BALB/c mice were infected orally with <i>B. pseudomallei</i> strain Bp1026b (5×10⁵ CFU), Bp2671a (2.0×10⁴ CFU), Bp2685a (4.8×10⁴ CFU) or Bp2719a (2.8×10⁴ CFU). Stomach tissues were collected from Bp1026b mice 56 days after infection, Bp2671a mice 21 days after infection, Bp2685a mice 3 days after infection, and Bp2719a mice 4 days after infection. All tissues were fixed in 10% NBF and embedded in paraffin before sectioning. FISH was performed on stomach tissue sections from mice infected with Bp1026b (A), Bp2671a (B), Bp2685a (C) and Bp2719a (D) as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0037324#s4" target="_blank">materials and methods</a>. Tissues were counterstained with DAPI (blue), and observed at 1000× final magnification. Tissue sections were hybridized with a eubacterial probe (green) and two <i>B. pseudomallei</i> specific probes (red). (E) The location of 1000× fields positive for <i>B. pseudomallei</i> was determined for each <i>B. pseudomallei</i> isolate. Positive 1000× fields are indicated by red (Bp1026b), yellow (Bp2671a), green (Bp2685a) or blue (Bp2719a) shaded outlines. Outlines were overlaid onto a representative stomach image created by combining images from a hematoxylin and eosin stained section. The esophagus (esoph.), body (corpus) and pylorus of the stomach are labeled for reference. In (A–D) the scale bar represents 10 microns, and in (E) the scale bar represents 2 mm.