Identification of Tomato necrotic stunt virus (ToNSV) in the Mexican sample (MX) and its relationship with other viruses in the family of Potyviridae.

<p>A. Relative placement of <i>de novo</i> assembled siRNA contigs onto a general potyvirus genome. B. Distribution of siRNAs along the ToNSV genome in both (+) and (−) polarities. C. Phylogenetic relationship of the newly identified ToNSV genome in relationship to other viruses in the family of Potyviridae. The full name of potyviruses and their respective GenBank accession numbers are: ToNSV-MX9354: JQ314463; PTMV, <i>Peru tomato mosaic virus</i>: AJ516010; PVY-O, <i>Potato virus Y</i>: HQ912914; PeSMV, <i>Pepper severe mosaic virus</i>: AM181350; PeMoV, <i>Pepper mottle virus</i>: EU586135; PVV, Potato virus V: NC_004010; PepYMV, <i>Pepper yellow mosaic virus</i>: NC_014327; BiMoV, <i>Bidens mottle virus</i>: EU250214; WMV, <i>Watermelon mosaic virus</i>: DQ399708; PVMV, <i>Pepper veinal mottle virus</i>: FM202327; TVBMV, <i>Tobacco vein-banding mosaic virus</i>: EF219408; TVMV, <i>Tobacco vein mottling virus</i>: X04083; and TEV, <i>Tobacco etch virus</i>: EF470242. SPMMV, <i>Sweet potato mild mottle virus</i>: NC_003739, genus <i>Ipomovirus</i>, was used as an outgroup sequence to anchor the phylogenetic tree.