The presence of magnet increases the extent of ligation in bead-bead subcloning.

<p>Flow cytometry of fluorescent-labeled beads allows quantification of bead-bead ligations. Insert beads are red-labeled (Alexa488 label) and acceptor-vector beads are green-labeled (Alexa647 label). Successful bead-bead ligations appear at high FL-1 and FL-6 readouts (boxed). The insert is ITGA2b (1 350 bp) and the acceptor vector is pLenti1 (8 180 bp). (A) Ligation in the absence of magnet. Approximately 0.5% of acceptor beads are ligated. (B) Ligation in the presence of magnet. Approximately 7% of the acceptor beads are ligated, a 14-fold increase in extent of ligation.