Induction of apoptosis by overexpression of Tβ₁₀ in 2774 ovarian cancer cells.

<p>(A) Subconfluent 2774 ovarian cancer cells were maintained for 5 days in complete culture medium containing Ad.TERT.LacZ or Ad.TERT.Tβ₁₀ at an MOI of 10 or 100, and the MTT-bioassay was performed daily. (B) On the second day after viral infection, a FACS Annexin V/propidium iodide (PI) assay was performed according to the manufacturer’s (Clontech) protocol. Representative scatter plots are showing the distribution of annexin V- and PI-stained cells. In top panels, the X-axis (FL1-H) indicates annexin V–FITC fluorescence detected at 518 nm, and the Y-axis (FL2-H) indicates PI fluorescence detected at 620 nm. As the lower left (LL) quadrant indicates live cells, the upper left (UL) quadrants indicate necrotic cells, the upper right (UR) quadrats indicate late apoptotic cells, and lower right (LR) quadrant indicates early apoptotic cells (top panels), the results are expressed as a percentage of total cell numbers and are mean ± SE (bars) of three individual experiments. Significant differences from vehicle group are denoted by an asterisk (*), P<0.05; a double asterisk (**), P<0.01.