Autoreactivity of mAb CH07.

<p>A–D. Indirect immunofluorescence staining of HEp-2 cells was strongly positive for mAbs CH07 (A, diffuse nuclear and clumped peripheral staining pattern) and 2F5 (C, diffuse cytoplasmic and nuclear staining pattern); mAbs CH08 (B) and 17b (D) were negative. All images taken for 10 s except CH07 taken for 7 s. Size bars are 25 µm. E. Reactivity of mAb CH07 to 9,000 human proteins in a microarray was assessed, each spot represents binding to a single protein. Pooled human IgG (hIgG) was used to measure background binding. The X-axis represents antibody binding minus background. The Y-axis represents antibody binding divided by the background binding. Positive signals for specific proteins are indicated by labels.