Enhanced recruitment of inflammatory monocytes to the site of infection in LysM-PPARγ^KO mice.

<p>LysM- PPARγ^WT and LysM- PPARγ^KO mice were infected i.p. with <i>Listeria</i> (2×10⁴ CFU). (A) at indicated time points total numbers of CD11b⁺ Ly6C^high monocytes in the peritoneal cavity (PEC), liver and spleen were determined by FACS analysis (B–C) Expression of CCL2, CCL7 and CCR2 in the liver (B) and spleen (C) of infected mice as determined by qRT-PCR. (D) LSEC isolated from wild type C57BL/6 mice were incubated <i>in vitro</i> with sterile filtered supernatant peritoneal macrophages from LysM- PPARγ^WT and LysM- PPARγ^KO mice that were either infected (18 hrs) or left non-infected. The fold increase in expression of CD54 on the surface of LSEC determined by increase in mean fluorescence intensity by flow cytometry in response to contact with supernatant from infected vs non-infected peritoneal macrophages was determined after 24 hrs.