The density of macrophages/activated microglia in the lesion epicentre does not differ between injured wild-type and EphA4 knockout spinal cords.

<p>Transverse sections of hemisectioned spinal cord were immunostained with CD11b to label infiltrating macrophages and activated microglia. Examples from wild-type (A) and EphA4 knockout (B) spinal cords 4 days post-injury are shown; the square surrounds the CD11b+ lesions on the left hand side of the spinal cord. There was no difference between genotypes in the proportional area of CD11b immunoreactivity within the injury epicentre at 2, 4 or 14 days post-injury (D). Higher power images of CD11b+ staining (example in C) were used to determine the density of cells within the injury epicentre; no difference was seen between injured wild-type or EphA4 knockout mice at 2, 4 or 14 days post-injury (E). Images in A and B show DAPI nuclear counterstain. Results show mean ± SEM of <i>n</i> = 3 per genotype at 2 and 14 d.p.i. and <i>n</i> = 6 per genotype at 4 d.p.i. Scale bars: A, B = 500 µm; C = 50 µm. WT = wild-type, KO = knockout, d.p.i. = days post-injury.