Fluorescence quenching spectra of bis-ANS bound HSA in absence (top) and presence (the arrow indicating curves obtained with increasing concentration) of virstatin in phosphate buffer pH 7.2; [HSA] = 7.5 µM, [bis-ANS] = 5.0 µM and [virstatin] = 0.1 to 5.0 µM (with 10 increments of 0.1 µM, followed by another 5 of 1 µM).

<p>Fluorescence quenching spectra of bis-ANS bound HSA in absence (top) and presence (the arrow indicating curves obtained with increasing concentration) of virstatin in phosphate buffer pH 7.2; [HSA] = 7.5 µM, [bis-ANS] = 5.0 µM and [virstatin] = 0.1 to 5.0 µM (with 10 increments of 0.1 µM, followed by another 5 of 1 µM).