Sedimentation velocity analytical ultracentrifugation determined the oligomerization statue of the N-terminal domain.

<p>(A and C) The panels showed the sedimentation coefficient distributions <i>c(s)</i> of purified M1N from the 17.6-ml fraction at pH 7.4 (A), and from the 17.4-ml fraction at pH 5.0 (C). The data were acquired at a protein concentration of 1.2 mg/ml. (B and D) The molecular mass distribution derived from <i>c(s)</i> data of panel A and C, respectively.