<i>Dp(11)17/+</i> mice (red) have similar food intake and activity levels, but higher energy expenditure than WT mice (gray), which may be partially accounted for by the difference in expression levels of UCP1 in the BAT tissue.

<p>(A) <i>Dp(11)17/+</i> mice have similar amount daily food intake to WT mice after 4 wks of age, although they consume less food at 3 wks (*p = 0.001) and 4 wks (*p = 0.048). (B) VersaMax system (Accuscan Inc., Ohio) using the beam block technique implemented in home cages revealed no difference in horizontal activity level between <i>Dp(11)17/+</i> and WT animals. (C, D) Oxygen consumption measured using the CLAMS system (Columbus Ins., Ohio) for over three days documented higher energy expenditure of <i>Dp(11)17/+</i> mice in the light phases alone (*p = 0.0095) and during the entire day (*p = 0.044). (E, F) Respiratory exchange ratio (RER) measured using the CLAMS system for over three days again confirmed higher metabolic activity of <i>Dp(11)17/+</i> mice (*p = 0.00151). (G) Western blot for UCP1 expression in BAT tissue of three <i>Dp(11)17/+</i> and three WT mice with antibody AB3036 (Millipore). The same blot was normalized to actin blotting using MAB1501 (Millipore). (H) Normalized intensity of UCP1 signals in <i>Dp(11)17/+</i> vs. WT mice (17.13±10.21 vs. 4.74±2.05, p = 0.35). The measurements are from (A) 5–13 <i>Dp(11)17</i>/+ and 5–11 WT at different time points (B) to (F) 12 <i>Dp(11)17</i>/+ and 7–10 WT at 25–32 wks (G) 3 <i>Dp(11)17</i>/+ and 3 WT at 30 wks.