<i>tbc-8(tm3802)</i> mutants display no defects in synaptic vesicle trafficking and localization.

<p>The transport of synaptic vesicles to the axonal release sites in the DNC was visualized by VENUS-tagged RAB-3 (VENUS-RAB-3) (A) and GFP-tagged synaptobrevin (GFP-SNB-1) (B). No differences were observed in transport of both SV markers in <i>tbc-8(tm3802)</i> mutants. Scale bar represents 5 µm. Error bars = s.e.m. (ns, P>0.05; Student's t-test). (C) Electron microscopic analysis of <i>tbc-8(tm3802)</i> mutants revealed no obvious differences in the morphology of synapses, cell bodies and neuronal Golgi stacks compared to wild type. (D) The synaptic vesicle distribution relative to the presynaptic density at synapses of <i>tbc-8(tm3802)</i> mutants was similar to wild type worms (Student's t-test).