Loss of CCDC6 affects DSBs repair.

<p>(<b>a</b>) Detection of DSBs by PFGE. After 10 Gy IR exposure CCDC6-depleted (shCCDC6) and CCDC6-proficient (shCTRL) HeLa cells have been collected at different time points (1, 2, 4, 24 hours). Densitometric analysis of DSBs bands were plotted as percentage of total DNA. <b>(b</b>) The percentages of GFP positive cells, compared to controls, have been plotted on the histograms that are representative of three independent experiments. Error bars indicate the standard error mean. The anti-HA-I-Sce1 and anti-tubulin immunoblots are shown at bottom of the figure. <b>(c</b>) HeLa cells, bearing the doxycycline-inducible I-Sce1 DNA repair construct, have been transfected with control shRNAs (shCTRL) or sh-CCDC6 by Microporator MP-100 transfection system (Digital Bio, Korea). The CCDC6 protein depletion was assessed by western blot analysis for every rate of transfection (#1, #2, #3, #4). The percentages of protein expression, compared to controls, have been plotted on the histogram below.