CCDC6 in the genome stability control.

<p>(<b>a</b>) shCCDC6 and shCTRL HeLa cells were depleted of PP4c by shRNA (48 hours) and were exposed to 1 Gy of IR, as indicated (−/+). Phosphorylation of H2AX, PP4c, CCDC6, total H2AX and tubulin amount were revealed at IB of WCL. (<b>b</b>) In the cell extract of CCDC6-depleted clone #1 (shCCDC6) and control HeLa cells (shCTRL), after double thymidine block (TT block) and release in presence of 1 µM Etoposide at several time point, as indicated, phosphorylation levels of H2AX and of RPA2 were revealed with anti-pH2AX S139 and with anti-p-RPA2 by western blot. Anti-total H2AX and anti-total RPA were shown as loading control. (<b>c</b>) Schematic diagram of CCDC6 function in modulating PP4c activity on the phosphorylation status of H2AX in the DNA-damage response.