Apoptosis in the kidney of TLR2, TLR4 and MyD88 deficient mice subjected to CLP.

<p>(<b>a</b>) Histological analysis of apoptosis by immunohistochemistry of cleaved caspase 3 of control (a), WT (b), TLR2 ^−/− (c), TLR4 ^−/− (d) and MyD88 ^−/− (e) mice respectively, 24 hours after CLP . Results of a representative experiment with 5 animals per group. (<b>b</b>) Histological analysis of apoptosis by immunofluorescence to TUNEL of control (a), WT (b), TLR2 ^−/− (c), TLR4 ^−/− (d) and MyD88 ^−/− mice (e) respectively, 24 hours after sepsis. (<b>c</b>) Score of cleaved caspase-3 in kidney represented in figure A. (<b>d</b>) Score of TUNEL in kidney represented in figure B. (<b>e</b>) Gene expression of BCL-2 in the kidney 24 hours after CLP. The mRNA was normalized to HPRT expression and compared to normal group. Results of a representative experiment with 5 animals/group. Data shown as mean ± standard deviation (SD), * p<0.05 vs WT and ** p<0.01 vs WT.