c-Fos expression in the DG, CA1 and CA3 regions of the hippocampus.

<p>c-Fos immunohistochemical analysis was performed in the hippocampus of 3-month-old (young) and 22-month-old (aged) mice fed with the control diet or the LCω3 diet for 2 months and sacrificed 90 min after the spatial recognition acquisition session. (A) Representative images of c-Fos immunohistochemistry in the DG (left panel), the CA1 (central panel) and the CA3 region (right panel) of the hippocampus. Scale bar = 100 µm. (B) Quantification of c-Fos-positive cells was performed in the DG, the CA1 and the CA3 regions of the hippocampus. Data are presented as mean ± SEM. ** p<0.01. (C) Correlation between the number of c-Fos positive cells induced by the Y-maze task in the DG (left panel), the CA1 (central panel) and the CA3 (right panel) and the spatial recognition score. Pearson's correlation coefficients (r) and corresponding significance (p) are displayed within each correlation window. The number of c-Fos positive cells in the DG and CA1, but not in the CA3 regions of the hippocampus was positively correlated to the spatial recognition score.