PARP inhibitory effect on anthocyanin accumulation is associated genetically with PARP activity and based on transcriptional control.

<p>The <i>Col-0</i>, <i>PAP1-OX</i> and T-DNA insertion lines of the three <i>PARP</i> genes (<i>parp1</i>, <i>parp2</i>, <i>parp3</i>) were grown for 14 days at 80–100 µE, 22°C on MS medium with (+3 MB) or without (−3 MB) 0.2 mM of the PARP inhibitor 3-Methoxy-benzamide (3 MB) in the medium. A, the relative anthocyanin content of the <i>PAP1</i>-OX line is shown, combined from three independent experiments with three replicates in each experiment (n = 9). The reduction of ∼20% is not significant (P = 0.07) and is much smaller than the reductions after 14 days for oxidative (∼70%) or sucrose (∼50%) (see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0037287#pone-0037287-g003" target="_blank">Fig. 3B</a>). B, the mutant lines were subjected to two different conditions; control or oxidative stress (0.1 µM Paraquat). The relative anthocyanin content is shown. Data are combined from 3 independent experiments with two to four replicate plates in each experiment (n = 9). Asterisks indicate significant difference (P<0.05) of the mutant lines compare to the Col-0 grown in the same condition.