Notch signaling regulates mesenchymal transition of c-Kit+ and c-Kit- cells.

<p>Notch signaling was up-regulated by over-expressing NICD-GFP adenovirus or suppressed with γ-secretase inhibitor XXI (GSI). (<b>A</b>) NICD transfection efficiency was monitored by GFP expression. (<b>B</b>) Notch modulation was confirmed by a Western blot analysis with anti-NICD antibody and (<b>C</b>) by qRT-PCR measuring expression level of Hey1. (<b>D</b>) qRT-PCR expression analysis after Notch signaling modulation. Expression levels were normalized to the level of β-actin. Fold changes were calculated as a ratio of expression in experimental (NICD- or GSI-treated) group to the expression in control group, * p<0.05. (<b>E, F</b>) FACS analysis of c-Kit+ (<b>E</b>) and c-Kit- (<b>F</b>) cells after Notch signaling modulation. N = 5 per group, *, p<0.05 compared to control.