Ablation of the Slc10a2 gene lowers the levels of plasma s TGs but not cholesterol.

<p>(A) Total plasma cholesterol and TGs from wt, <i>Slc10a2+/−</i> and <i>Slc10a2−/−</i> mice. (B) Plasma profiles of cholesterol and TGs (C) were analysed from wt, <i>Slc10a2+/−</i> and <i>Slc10a2−/−</i> animals by fast protein liquid chromatography (FPLC). Lines represent means of all individuals from each group, respectively. Lipoprotein fractions are indicated, n = 9–10. (D) Hepatic 3-hydroxy-3-methyl-glutaryl-CoA (HMG-CoA) reductase mRNA levels and enzymatic activity of wt, <i>Slc10a2+/−</i> and <i>Slc10a2−/−</i> mice. HMG-CoA reductase enzymatic activity was analyzed from pooled hepatic microsomal samples and represents mean of two measurements. (n = 9–10). (E) Hepatic mRNA levels of the sterol transporters ATP-binding cassette sub-family G member 5 (Abcg5) and Abcg8 in wt, <i>Slc10a2+/−</i> and <i>Slc10a2−/−</i> mice. (F) Hepatic mRNA levels of sterol regulatory element-binding protein 1c (Srebp1c) and Srebp2, in wt, <i>Slc10a2+/−</i> and <i>Slc10a2−/−</i> mice. mRNA levels in wt mice were normalized to 1. Data are expressed as mean ± standard error (SEM) for the mRNA and total plasma cholesterol and TGs analyzes. wt littermates (n = 10), <i>Slc10a2</i>+/− (n = 9) and <i>Slc10a2</i>−/− (n = 10) for A-F. Significances of differences between groups was tested by Dunnetts test, a p-value <0.05 is denoted *.