Assembly and function of Mu transpososome core.

<p>This pathway is based on <i>in vitro</i> studies and utilizes a minimum number of macromolecular components <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0037922#pone.0037922-Savilahti1" target="_blank">[27]</a>. The <i>in vivo</i> assay described earlier <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0037922#pone.0037922-Pajunen3" target="_blank">[60]</a> and used here (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0037922#pone.0037922.s001" target="_blank">Figure S1</a>) is a close mimic of this minimal-component <i>in vitro</i> system with regard the following features: (i) the configuration includes two MuR-ends (with R1 and R2 MuA binding sites), (ii) the phage-encoded MuB protein and (iii) transpositional enhancer are not included. See <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0037922#pone.0037922.s001" target="_blank">Figure S1</a> for a full description of Mu transposition pathway and its comparison to the pathway used in the papillation analysis. The R1 and R2 MuA binding sites are shown as rectangles. MuA is drawn as a tetramer of yellow circles and target DNA is shown in purple. The small arrows on the target DNA indicate the 5-bp staggered locations for strand transfer on the two strands. The dots in the assembled transpososome indicate the Mu end cleavage sites.