Detection of prostaglandin synthase activity of rFhGST-S1 via a mass spectrometry approach.
- E. James LaCourse (162749)
- Samirah Perally (162750)
- Russell M. Morphew (162753)
- Joseph V. Moxon (162120)
- Mark Prescott (128014)
- David J. Dowling (162757)
- Sandra M. O'Neill (162760)
- Anja Kipar (87443)
- Udo Hetzel (162762)
- Elizabeth Hoey (162765)
- Rafael Zafra (162768)
- Leandro Buffoni (162770)
- José Pérez Arévalo (162774)
- Peter M. Brophy (162777)
Publication date
May 2012
DOIAbstract
<p>A coupled assay with rFhGST-S1 and COX-1 catalyses the conversion of arachidonic acid to the H2 form before the prostaglandin isomer is converted to either the D or E form. Nano-LC/MS analysis allowed detection of both PGD2 (A) and PGE2 (B) in the assay mixture with the PGD2 form being the more abundant of the two prostanoids (C). Boxed figures above peaks show the fragmentation ions specific to detection of PGD2 (a) and PGE2 (b) according to the method of Schmidt <i>et al. </i><a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0001666#pntd.0001666-Schmidt1" target="_blank">[39]</a>.</p
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