Assessment of cytosolic Ca²⁺ signaling in MDA-MB-468 and MDA-MB-231 breast cancer cell lines.

<p>Average ATP-stimulated Ca²⁺ entry response (100 µM ATP), store-operated Ca²⁺ entry response (10 µM CPA) and non-stimulated Ca²⁺ influx (DMSO control) were assessed in <b>A</b>) MDA-MB-468 and <b>B</b>) MDA-MB-231 cells. Quantitation of peak relative Ca²⁺ influx (peak 2) in <b>C</b>) MDA-MB-468 and <b>D</b>) MDA-MD-231 cells; shown as average ± S.D. (n = 9 for MDA-MB-468 & n = 4 for MDA-MB-231 cells). <b>E</b>) MDA-MB-468 cells exhibit spontaneous and asynchronous Ca²⁺ oscillations that are attenuated by extracellular Ca²⁺ chelation (BAPTA). <b>F</b>) Still image showing the Y-plane section and <b>G</b>) the Y-plane projection through time. See also <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0036923#pone.0036923.s001" target="_blank">Movie S1</a>. Representative of five movies from three independent experiments.