Establishment of a feline fibrosarcoma cell line.

<p>(A) Hematoxylin & Eosin staining of the pathologic specimen. Optical microscope 100× magnification of the neoplastic tissue shows cells that appear to have indistinct cell borders, round to oval nuclei with finely stippled chromatin, and small single or multiple nucleoli. (B–F) Phase contrast imaging of the Cocca-1A, -2A, -3A, -6A, and -3B clones after 48 hours of culture (200× magnification). (G) Phase contrast, 200× magnification of the clone 6A after 3 months of continuous culture. (H–J) Phase contrast, 200× magnification of the clone 6A after 6-, 12-, and 24 months of continuous culture.