COUP-TFII increases <i>RARβ2</i> transcription in MCF-7 cells.

<p>A, ChIP of COUP-TFII-FLAG to the <i>RARB2</i> promoter in MCF-7 cells transfected with empty vector (EV) or COUP-TFII (CII) and treated with EtOH or 1 µM atRA for 6 h. * P<0.05 <i>versus</i> E control, <i>‡</i> P<0.05 <i>versus</i> CII-EtOH. B-C, Cells were transfected with parental or expression plasmids for COUP-TFII or nucleolin for 24 h and were treated with EtOH, 10 µM CRO or AS1411 for 24 h post-transfection. For C, cells were transfected with 0.5 µg and 1 µg of nucleolin and COUP-TFII expression vector, respectively. Q-PCR was performed to determine <i>RARB2</i> expression. Values are the average of 6 separate experiments ± SEM. D, Cells were transfected with pcDNA or pcCOUP-TFII and treated with 10 µM RO or AS1411 for 24 h. Dual luciferase activity was expressed relative to the pcDNA-transfected, no-treatment control. Values are mean ± S.E.M. of two separate experiments. For B–D, * P<0.05 <i>versus</i> vector control, ** COUP-TFII alone, or ▴ between the indicated values.