Identification of NSCs and protoplasmic astrocytes <i>in vitro</i>.

<p>(A) Representative photomicrograph of neurospheres in culture. (B) Immunocytochemical staining of purified NSCs with Nestin(bar = 82.91 µm). (C) Immunocytochemical staining of NSCs with anti-Brd-U antibody (bar = 48.93 µm). (D) Immunocytochemical staining of differentiated cells from NSCs (green indicates neuron-specific label β-tubulin; red indicates the astrocyte-specific label GFAP; blue indicates the nucleus-specific label DAPI; bar = 75 µm). (E) Representative photomicrograph of purified protoplasmic astrocytes (×100). (F) Immunocytochemical staining of purified protoplasmic astrocytes with GFAP. (G) Nucleus staining of purified protoplasmic astrocytes with DAPI. (H) Merge of F and G. The scale bar = 37.5 µm for F, G, and H.