Validation of the specificity of miR-132 sponge vectors and experimental diagram.

<p>(<b>A</b>) Schematic of vectors encoding GFP containing 20 tandem, miR-132-binding sites in its 3′UTR (orange bars) to “sponge” out miR-132 (132-SP) and a control vector encoding mutant GFP containing 20 random sites in its 3′UTR (SCR9-SP). (<b>B</b>) Schematic of a sensor vector encoding RFP containing perfectly complementary miR-132 binding sites (blue bars). (<b>C</b>) Confocal images of Neuro-2A cells transfected with the sensor vector and SCR-SP or 132-SP. Scale bar: 70 µm. (<b>D</b>) Diagram of our experimental paradigm. DNA constructs were introduced into the lateral ventricle of P0–P1 pups for electroporation into neural progenitor cells. 4–6 weeks post-electroporation, fluorescently tagged, synaptically integrated newborn neurons were analyzed.