Cathepsin B is released from lysosomes in HIV-infected MDM.

<p>To analyze the lysosomal localization of cathepsin B, cathepsin B and LAMP2 immunoreactivity were assessed by <i>in situ</i> PLA (Duolink) in uninfected and HIV-infected MDM 3, 6 and 12 dpi. Cathepsin B colocalizes with LAMP2 in uninfected MDM (A, B and C; top panels). However, little colocalization is seen in HIV infected MDM (D, E and F; bottom panels). The presence of individual proteins was determined by immunofluorescence staining (G, H, I, J, K and L). As seen in the right panels both, cathepsin B (red) and LAMP2 (green) are expressed in uninfected (G, H, and I) and HIV-infected (J, K, L) cells. The results presented in this figure are representative of 3 experiments.